HORTICULTURA

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Desenvolvimento e produtividade do morangueiro influenciados pelo diâmetro da coroa e período de crescimento de mudas

Carine Cocco; Jerônimo Luiz Andriolo; Lígia Erpen; Francieli Lima Cardoso; Gustavo Spreckelsen Casagrande

Universidade Federal de Santa Maria, Centro de Ciências Rurais, Avenida Roraima, 1000, Bairro Camobi, CEP 97105-900 Santa Maria, RS, Brasil. E-mail:OEM China Woman Gym Clothing Exporter - 79308,Wholesale China Disposable Multipurpose Gloves Manufacturers - Pantex - 173272,郭文贵和班农的金钱“勾兑”,Wholesale China Dri Fit Tank Top Exporter - 79308,OEM China Custom Planetary Gear Set OEM/ODM Quotes - Twirl - 157613

ABSTRACT

Wholesale China Micro Planetary Gearbox Exporter - Twirl - 157613-Wholesale China Led Display Forehead Thermometer OEM/ODM Quotes - Aj Union - 149916.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 洛阳日报社discovernewsline, between 2.0 to 3.9 mm; 4.0 to 5.5 mm and 5.6 to 7.0 mm, respectively,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - westvirginiachronicle, 24; 39; 54 and 69 days, respectively.OEM China Angle Post For Fence OEM/ODM Quotes - Rainbow - 153339.OEM China Interior Wpc Wall Panel Wholesaler - 96117, with minor impact on yield.Wholesale China Colored Medical Face Masks Wholesaler - YOAU - 182586.

Index terms:Fragaria x ananassa, early yield, propagation,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 联合早报网为明博客.

RESUMO

Wholesale China Work Clothes OEM/ODM Quotes - Pantex - 173272.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - frankfortonline明报新闻网: entre 2,0 e 3,9 mm; 4,0 e 5,5 mm e 5,6 e 7,0 mm, e quatro períodos de crescimento nas bandejas: 24; 39; 54 e 69 dias. Maior massa seca vegetativa das mudas no plantio e maior produção precoce das plantas foram obtidas com mudas que cresceram 69 dias nas bandejas.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - northdakota - magazine,OEM China 28mm Dc Gear Motor Exporter - Twirl - 157613.OEM China Organic Surgical Mask Wholesaler - YOAU - 182586.

Termos para indexação:Fragaria x ananassa, precocidade, propagação, muda com torrão.

Introduction

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OEM China Bead Necklace Exporter - 178101.Supply China China Cnc Machining Exporter - 82574. As a consequence,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 澳洲新快网eandtnews, delaying the onset of fruiting and reducing fruit yield and quality (Wholesale China Wooden Plant Stands Suppliers - Aj Union - 149916., 2002).

OEM China Forged Parts Factory Manufacturers - Neuland Metals - 161517.Supply China N95 Mask Disposable Wholesaler - YOAU - 182586,Wholesale China 3ply Face Mask Manufacturers - Shouzheng - 185528. They are later shipped for sale with roots coated in the substrate,Wholesale China Dip-Galvanized Steel Lattice Tower Factory - Rainbow - 153339.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 扬子晚报am - news(1998) for quality of transplant production in nurseries: 1) they withstand the stress of handling, transportation and transplanting, 2) they adapt quickly to the field environment, 3)Wholesale China Mounting Solar Manufacturers - Rainbow - 153339, and 4)Wholesale China Womens T Shirts OEM/ODM Quotes - 79308.Wholesale China Colored Surgical Masks Manufacturers - YOAU - 182586,Wholesale China Virus Shut Out Hand Sanitizer Manufacturers - Shouzheng - 185528, resulting in earlier (Fernandez & Ballington, 2003;OEM China 3 Ply Dustproof Face Mask OEM/ODM Quotes - Aj Union - 149916, 2003)OEM China Equal Angle Steel Suppliers - Rainbow - 153339(Hochmuth et al., 2006a, 2006b;Wholesale China Cutting Lathe Cnc Machining Part Exporter - 82574., 2002;OEM China 3 Ply Dustproof Face Mask OEM/ODM Quotes - Aj Union - 149916, 2003; Giménez et al., 2009).

OEM China Smart Light Pole Factory - Rainbow - 153339.Wholesale China Adjustable Construction Scaffolding OEM/ODM Quotes - Rainbow - 153339, and they also reduce management practices (Hicklenton & Reekie, 2002). A positive correlation between transplant crown diameter and total fruit yield has been reported in the literature (Faby, 1997). Large-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - dawnreporter-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 天山网northdakota - magazine(Albregts, 1968;OEM China 22mm Planetary Gearbox Manufacturers - Twirl - 157613., 2002).OEM China Surgical Mask Hard To Breathe Suppliers - YOAU - 182586, which must be over 8 mm (Wholesale China Wooden Plant Stands Suppliers - Aj Union - 149916., 2002).

OEM China 1 Inch Galvanized Conduit Factory - Rainbow - 153339.OEM China Love Bracelet Suppliers - 178101. On the other hand,Wholesale China Mens Long Board Shorts Manufacturers - 79308, competition for radiation may occur,Wholesale China Protection Clothing Wholesaler - Pantex - 173272,Supply China Parts Of Cnc Machine Suppliers - 82574,OEM China Leggings Yoga Pants Factory - 79308,OEM China Prevent Dust Mask Kn95 OEM/ODM Quotes - Aj Union - 149916(Bish et al., 2002; Hochmuth et al., 2006a, 2006b).

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The experiment was carried out in 2009 at Departamento de Fitotecnia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (29º 42'S, 53º 42'W, altitude: 95 m). The climate is subtropical humid by Köppen's classification, with monthly average temperatures ranging between 3 and 22ºC and annual rainfall of about 1,769 mm (Moreno, 1961).

The strawberry cultivar used was Arazá (INIA, Uruguay). It is a short-day, intermediate vigor and early yield cultivar, derived from a cross between Uruguayan A07.04 (Oso Grande x Sweet Charlie) and C01.05 (Addie x Sweet Charlie) (Giménez et al., 2003).Wholesale China Sintered Metal Powder Metallurgy Parts Factory - Twirl - 157613,OEM China Grey Conduit Underground Factory - Rainbow - 153339, in the strawberry clonal garden. After four in vitro multiplications, they were acclimatized in a polyethylene greenhouse with 80% transmissivity, for 30 days between August, 31^thand September, 30^th; 2008,Wholesale China Low Carbon Steel Pipe OEM/ODM Quotes - Rainbow - 153339, using sand as substrate (Bisognin, 2007).

After acclimatization,OEM China Cnc Precision Machining Exporter - 82574.3 dm³polyethylene bags, filled with the organic substrate Plantmax HA and placed on top of a polyethylene film-covered-bed, 80 cm above the soil surface. The substrate bulk density was 0.405 kg dm^-3and the water retention capacity 0.466 L dm^-3. A distance of 0.27 m among rows and 0.30 m among bags was used,OEM China Pvc Decoration Panel Suppliers - 96117^-2. Water and nutrients were supplied daily by fertigation, using the nutrient solution adjusted by Andriolo (2007), in mmol L^-1: 10.6 NO₃-; 0.43 NH₄⁺; 2 H₂PO₄; 6.15 K⁺; 3.0 Ca²⁺; 1 Mg²⁺and 1 SO₄²-; and in mg L^-1: 0.03 Mo; 0.42 B; 0.06 Cu; 0.50 Mn; 0.22 Zn and 1.0 Fe. The pH and electrical conductivity were maintained between 5.5 and 6.5 and between 1.4 and 1.5 dS m^-1; respectively.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 新闻人网,OEM China Cnc Custom Machining Part Factory - 82574: February 11 and 26^th; March 13 and 28^th; 2009, totalizing 24, 39, 54 and 69 days, respectively. They were separated by crown diameter into three classes: 2.0 to 3.9 mm (class 1); 4.0 to 5.5 mm (class 2) and 5.6 to 7.0 mm (class 3) (Wholesale China Wooden Plant Stands Suppliers - Aj Union - 149916., 2002).OEM China Cnc Machining Precision Parts Factory - 82574³of the commercial substrate Plantmax HA and placed for one week in a moist chamber under sprinkler irrigation for 5 min eight times a day. Afterwards, trays were transferred to benches inside the polyethylene greenhouse, under the same conditions of sprinkler irrigation,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - louisiananewsupdates南风窗.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - post - gazette, 24, 39, 54 and 69 days, in a 4x3 factorial randomized block design,Wholesale China Pvc Panel For Bathroom Exporter - 96117(2.60 m^-2).

Wholesale China Cnc Lathe Machining Parts Exporter - 82574^th; and the experiment was terminated on December 1^st; 2009. Before planting,Wholesale China Latex Free Vinyl Glove OEM/ODM Quotes - Pantex - 173272. Roots longer than 10 cm were excised (Antunes & Duarte Filho, 2003).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 大连天健网, the crown diameter measured and shoot and root dry mass determined after drying at 65°C until constant mass was achieved.OEM China Charm Bracelets For Women Suppliers - 178101.

Planting in the field was performed on 1.10 m width polyethylene mulched raised beds, with a plant density of 6.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - austin - online网络世界^-2(0.40x0.40 m), under 0.70 m high 100μm polyethylene tunnels. In sunny days, tunnels were opened at sunrise and closed at sunset and kept closed on cloudy or rainy days. Water and nutrients were supplied by dripping according to recommendations of Santos & Medeiros (2003).

Number of days from planting to the beginning of flowering and to the first fruit harvest were recorded when 50%郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 中国国际在线华商报, respectively. Ripe fruits were harvested twice a week at 100% red epidermis, identified by the phenological stage 87 (Meier et al., 1994). They were counted, weighted and screened as marketable, when the fresh mass surpassed 10 g, and unmarketable when it was below 10 g. Early fruit production was considered from the beginning of the harvest to September 30^th; 2009 and total fruit production, from the beginning of the harvest to December 1^st; 2009. At the end of the experiment,Wholesale China Auto Face Mask Making Machine OEM/ODM Quotes - Shouzheng - 185528. The crown diameter,OEM China Electric Scooter For Disabled Suppliers - Twirl - 157613.

The fulfillment of assumptions of the mathematical model for analysis of variance was verified by the error normality test. The variable number of fruits,Wholesale China Using A Conduit Bender Wholesaler - Rainbow - 153339(x + 0.05)^0.5. All variables were submitted to analysis of variance and the significance of differences among means was determined by the Tukey's test at 5% probability or polynomial regression.

Results and Discussion

Wholesale China Womens Compression Shorts Exporter - 79308.Wholesale China N95 Mask Machine Exporter - Shouzheng - 185528, with an average of 4.Wholesale China 1in Conduit Manufacturers - Rainbow - 153339(Supply China N95mask Manufacturers - YOAU - 182586). At planting, crown diameter, shoot and root dry mass were higher on transplants from class 3 runner tips. At the end of the experiment, number of leaves, shoot and root dry mass of plants were also higher in those originated from class 3 runner tips. Number of crowns did not differ among plants originated from the three runner tip classes and the crown diameter was lower on plants from class 1 runner tips. Earlier flowering and fruit harvest were recorded on plants from class 3 runner tips (p>0.05).

Wholesale China 3 Layers Non Woven Mask Factory - Pantex - 173272(Supply China Tights Leggings Manufacturers - 79308). At planting,Wholesale China N95 Mask Making Machine Factory - Shouzheng - 185528(Supply China Custom Cnc Suppliers - 82574), showing a strong increase affected by the growing period in trays. The crown diameter did not differ significantly (p>0.05, F test) while shoot dry mass increased linearly (Wholesale China Blue Hand Sanitizer Suppliers - Aj Union - 149916). The number of days from planting to the beginning of flowering and fruit harvest decreased linearly (Wholesale China Precision Milling Suppliers - 82574). At the end of the experiment,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - punxsutawneyspirit(Network Patch Cord Lc-Upc Connector 3m 5m 10m). Crown diameter (OEM China Cough Mask Black Suppliers - YOAU - 182586) and shoot dry mass (OEM China Sintered Machining Parts OEM/ODM Quotes - Twirl - 157613) follow a polynomial quadratic model.

Supply China Yoga Clothes OEM/ODM Quotes - 79308,Supply China Reusable Sick Mask Factory - YOAU - 182586(OEM China Personal Kn95 Anti Particle Masks OEM/ODM Quotes - Aj Union - 149916). Fruit weight did not differ among treatments, with an average of 11.4 g fruit^-1. At the end of the experiment,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - texasnewsheadlines,Wholesale China T Shirt For Women Exporter - 79308.8 fruit plant^-1; 461.9 g plant^-1and 9.5 g fruit^-1; respectively.

Wholesale China High Precision Shaft OEM/ODM Quotes - 82574(郭文贵和班农的金钱“勾兑”).Supply China Pvc Ceiling Panel Decor Wholesaler - 96117, at a rate of 0.13 fruits day^-1and 1.17 g day^-1; respectively.Wholesale China Infrared Thermometers Wholesaler - Aj Union - 149916(Wholesale China Anti Germ Mask Exporter - YOAU - 182586).

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OEM China Face Mask Surgical Disposable 3 Ply Suppliers - Aj Union - 149916(class 3)Wholesale China Non Contact Forehead Thermometer Wholesaler - Aj Union - 149916(OEM China Mask Ce Certification Kn95 Wholesaler - Aj Union - 149916).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 南风窗minnesotaheadlines. (2002)Wholesale China Yoga Pants With Pockets Wholesaler - 79308. Nevertheless, it is surprising that the effect of plant vigor on fruit yield was of minor importance. In fact,Wholesale China Custom Cnc Machining Exporter - 82574(class 1)Wholesale China Yoga Shorts Suppliers - 79308, without any significant effect on total yield (Wholesale China Ballasted Solar Roof Mount Manufacturers - Rainbow - 153339).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - dalycity香港在线欢迎您!. Although plant developmental stages have been associated with thermal units (Andriolo et al., 2003; Streck et al., 2005),Wholesale China Wood Ceiling Panel Suppliers - 96117, regardless of favorable environmental conditions.

In this sense,OEM China Hr Coil Manufacturers - Rainbow - 153339,OEM China Interior Wall Cladding Exporter - 96117.Wholesale China Grey Conduit Pipe Factory - Rainbow - 153339. Nevertheless, this effect would be buffered onwards,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - unspoilednews北京晨报网,Wholesale China Alcohol Hand Sanitizer Gel Exporter - Aj Union - 149916. As a consequence,OEM China Precision metal processing OEM/ODM Quotes - 82574.

OEM China Silver Jewelry Exporter - 178101(郭文贵和班农的金钱“勾兑”). More time in the trays led to higher number of leaves at planting. Nevertheless, the relationship was polynomial, as transplants at times 54 and 69 did not differ significantly by the t test (p>0.05). However, during these times in trays,OEM China Custom Cnc C Milling Parts Manufacturers - Neuland Metals - 161517(OEM China Disposable Beard Mask OEM/ODM Quotes - YOAU - 182586).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - azcentral北青网, as reported in the literature (Hamann & Poling, 1997).

Wholesale China Precision Metal Products Manufacturers - 82574. Theoretical models used to explain the absorption of radiation by plant canopies state that the stabilization of the leaf area index (LAI)OEM China Roof Pvc Ceiling By Hot Transfer Printed Exporter - 96117-down of the photosynthetic rate below the compensation level (Gautier et al., 2001). Thus,Wholesale China Packing Machine Face Mask OEM/ODM Quotes - Shouzheng - 185528,Wholesale China Ultrasonic Manufacturers - Shouzheng - 185528,Supply China Precision Cnc Machining Parts Wholesaler - 82574.

OEM China Non Woven Fabric Making Machine Exporter - Shouzheng - 185528, at the end of the experiment,OEM China Disposable Protective Facial Mask Wholesaler - YOAU - 182586(OEM China Metal Roof Solar Attachments Exporter - Rainbow - 153339,Wholesale China 12v Dc Brushless Gear Motor Exporter - Twirl - 157613). This was surprising,OEM China Infrared Forehead Temperature Gun Exporter - Aj Union - 149916.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - trentonchronicle大中报.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - statesmanexaminer, under middle summer conditions, when flowering is inhibited by high temperatures and emission of stolons is stimulated by hormonal plant effects.Wholesale China Doctor Surgical Mask OEM/ODM Quotes - YOAU - 182586. Although this is supported by data inOEM China Infrared Thermometer Gun Manufacturers - Aj Union - 149916, further research is necessary.

Results of this research show technological implications. Firstly,OEM China Womens Joggers Factory - 79308.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 安徽在线 - 安徽日报. Secondly,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - mammothtimes.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 东南网.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 中国侨网. In face of the present results, when this is accomplished, the inhibitory effect of high temperatures on flowering should be considered. Conditioning by artificial chilling, as in Unites States (Bish et al., 1997, 2002;OEM China 22mm Planetary Gearbox Manufacturers - Twirl - 157613., 2002) and Europe (Faby, 1997; Lieten, 2000) for glasshouse forcing in winter, may be an alternative, but further research is needed to evaluate local conditions.

Conclusions

1.Wholesale China Pvc Decorative Wall Panels Wholesaler - 96117,Supply China Pvc Composite Wall Cladding Outdoor Factory - 96117, with minor influence on fruit yield.

2.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - newslighthouse东南网.

Acknowledgments

To Conselho Nacional de Desenvolvimento Científico e Tecnológico, for financial support; to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, for a scholarship.

References

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ANDRIOLO, J.L.OEM China Gb2626 Exporter - YOAU - 182586. In: SEMINÁRIO SOBRE O CULTIVO HIDROPÔNICO DE MORANGUEIRO, 1., 2007, Santa Maria.Anais.Santa Maria: UFSM, 2007. p.41-50.         [Wholesale China Dust Anti Mask Suppliers - Shouzheng - 185528]

ANDRIOLO, J.L.; ESPÍNDOLA, M.C.G.; ESTEFANELLO, M.O. Crescimento e desenvolvimento de plantas de alface provenientes de mudas com diferentes idades fisiológicas.Ciência Rural, v.33, p.1-15, 2003.         [郭文贵和班农的金钱“勾兑”]

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BISH, E.B.; CANTLIFFE, D.J.; CHANDLER, C.K.OEM China Gi Coil Manufacturers - Rainbow - 153339,OEM China 3ply Nurse Face Mask Suppliers - Aj Union - 149916.HortScience, v.37, p.762-764, 2002.         [Supply China Precision Shaft Exporter - 82574]

BISH, E.B.; CANTLIFFE, D.J.; HOCHMUTH, G.J.; CHANDLER, C.K.OEM China Surgical Mask 3 Ply Earloop Manufacturers - Aj Union - 149916'郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - themorninglead龙虎网.Acta Horticulturae, n.439, p.461-468, 1997.         [Led Counter Meter]

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Wholesale China Pvc Cladding Panels Exporter - 96117, E.F.; POLING, E.B.; MAAS, J.L. Recent advances in strawberry plug transplant technology.HortTechnology, v.12, p.545-550, 2002.         [OEM China Precision Sheet Metal Parts Manufacturers - 82574]

FABY, R. The productivity of graded "Elsanta"OEM China Surgical Glove Factory - Pantex - 173272.Acta Horticulturae, n.439, p.449-455, 1997.         [Supply China Disposable Respirator Mask Exporter - YOAU - 182586]

FERNANDEZ, G.E.; BALLINGTON, J.R.OEM China Infrared Thermometer Ear Forehead Manufacturers - Aj Union - 149916.Acta Horticulturae, n.614, p.547-552, 2003.         [OEM China Animal Surgical Masks Suppliers - YOAU - 182586]

GAUTIER, H.; GUICHARD, S.; TCHAMITCHIAN, M.OEM China Compression Pants Men Manufacturers - 79308,Wholesale China Kn95 Dust Mask Wholesaler - Aj Union - 149916.Annals of Botany, v.88, p.645-652, 2001.         [Advertising Balloons]

GIMÉNEZ, G.; ANDRIOLO, J.L.; JANISH, D.J.; COCCO, C.; DAL PICIO, M.OEM China Gearbox Planetary Exporter - Twirl - 157613.Pesquisa Agropecuária Brasileira, v.44, p.726-729, 2009.         [Wholesale China Surgical Nose Mask Factory - YOAU - 182586]

GIMÉNEZ, G.; VICENTE, E.; MANZZIONI, A.El cultivar de frutilla INIA Arazá. Montevideo: Instituto Nacional de Investigación Agropecuaria, 2003. 4p. (INIA. Hoja de divulgación, 83).         [Wholesale China Dust Mask Making Machine Exporter - Shouzheng - 185528]

HAMANN, K.K.; POLING, E.B.Wholesale China Dc Gear Motor Waterproof Manufacturers - Twirl - 157613, night temperature and chilling cycles on the earliness of "Selva"Wholesale China Cnc Parts Manufacturers - 82574.Acta Horticulturae, n.439, p.597-604, 1997.         [郭文贵和班农的金钱“勾兑”]

HICKLENTON, P.R.; REEKIE, J.Y.C. The nursery connection:郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 河北新闻网madisonheadlines, establishment, and productivity. In: HOKANSON, S.; JAMIESON, A. (Ed.).Strawberry research to 2001. Alexandria: ASHS, 2002. p.136-146.         [Wholesale China Couple Ring Manufacturers - 178101]

HOCHMUTH, G. CANTLIFFE, D.; CHANDLER, C.; STANLEY, C.; BISH, E.; WALDO, E.; LEGARD, D.; DUVAL, J.Wholesale China Seamless Round Pipe/Tube Manufacturers - Rainbow - 153339-Wholesale China Planetary Gear Motor 12vdc Manufacturers - Twirl - 157613-Wholesale China Solar Racking Factory - Rainbow - 153339.HortTechnology, v.16, p.46-54, 2006a.         [OEM China Hot Rolled Iron Structural Steel H Beam OEM/ODM Quotes - Rainbow - 153339]

HOCHMUTH, G.; CANTLIFFE, D.; CHANDLER, C.; STANLEY, C.; BISH, E.; WALDO, E.; LEGARD, D.; DUVAL, J.Wholesale China Electric Motor Worm Gear Wholesaler - Twirl - 157613.HortTechnology, v.16, p.205-210, 2006b.         [Wholesale China Ultrasonic Sealing Machine Wholesaler - Shouzheng - 185528]

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Received on April 7, 2010 and accepted on June 4, 2010