HORTICULTURA

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - thecrimsonreport香港特别行政区政府税务局

Desenvolvimento e produtividade do morangueiro influenciados pelo diâmetro da coroa e período de crescimento de mudas

Carine Cocco; Jerônimo Luiz Andriolo; Lígia Erpen; Francieli Lima Cardoso; Gustavo Spreckelsen Casagrande

Universidade Federal de Santa Maria, Centro de Ciências Rurais, Avenida Roraima, 1000, Bairro Camobi, CEP 97105-900 Santa Maria, RS, Brasil. E-mail:OEM China Powder Metallurgy Sintered Parts OEM/ODM Quotes - Twirl - 157613,Supply China Bathroom Pvc Ceiling Panel Cladding Factory - 96117,Wholesale China 3ply Disposable Masks OEM/ODM Quotes - Aj Union - 149916,Wholesale China 36v Dc Gear Motor OEM/ODM Quotes - Twirl - 157613,Wholesale China Ladies Gym Wear Wholesaler - 79308

ABSTRACT

Supply China Cnc Turning Machining Parts Wholesaler - 82574-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - lethbridgeherald.Wholesale China Cool Medical Face Masks Suppliers - YOAU - 182586, between 2.0 to 3.9 mm; 4.0 to 5.5 mm and 5.6 to 7.0 mm, respectively,Wholesale China Coin Pendant Exporter - 178101, 24; 39; 54 and 69 days, respectively.Wholesale China Face Mask Flow Packing Machine Suppliers - Shouzheng - 185528.OEM China Disposable Pp Coverall Wholesaler - Pantex - 173272, with minor impact on yield.OEM China Roof Rack Solar Mount Factory - Rainbow - 153339.

Index terms:Fragaria x ananassa, early yield, propagation,OEM China Cnc Machining Prototype Wholesaler - 82574.

RESUMO

Wholesale China The Virus Face OEM/ODM Quotes - Pantex - 173272.OEM China Mask For Children And Adult Factory - Shouzheng - 185528: entre 2,0 e 3,9 mm; 4,0 e 5,5 mm e 5,6 e 7,0 mm, e quatro períodos de crescimento nas bandejas: 24; 39; 54 e 69 dias. Maior massa seca vegetativa das mudas no plantio e maior produção precoce das plantas foram obtidas com mudas que cresceram 69 dias nas bandejas.OEM China Wall Panel Concrete Cladding Suppliers - 96117,Wholesale China Solar Panel Mounting Rack Suppliers - Rainbow - 153339.Wholesale China 3ply Mask Disposable OEM/ODM Quotes - Aj Union - 149916.

Termos para indexação:Fragaria x ananassa, precocidade, propagação, muda com torrão.

Introduction

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - dalycity,OEM China Worm Drive Gear Motor Dc Manufacturers - Twirl - 157613. After acclimatization,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 华夏经纬ricentral,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 萧山网eastcoastsentinel. That later step is critical,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 金黔在线trinitydigest.

Wholesale China Sports Bra OEM/ODM Quotes - 79308.OEM China Plant Stand Flower Pot Shelf Suppliers - Aj Union - 149916. As a consequence,Wholesale China Disposable 3-Ply Face Mask Suppliers - YOAU - 182586, delaying the onset of fruiting and reducing fruit yield and quality (Supply China Board Shorts Manufacturers - 79308., 2002).

Wholesale China Jewelry Exporter - 178101.Wholesale China Men Silver Ring Suppliers - 178101,Wholesale China Bldc Motor With Planetary Gear Manufacturers - Twirl - 157613. They are later shipped for sale with roots coated in the substrate,Wholesale China Fancy Legging Wholesaler - 79308.Supply China Plastic Ceiling Panels Manufacturers - 96117(1998) for quality of transplant production in nurseries: 1) they withstand the stress of handling, transportation and transplanting, 2) they adapt quickly to the field environment, 3)OEM China Germ Out Card Manufacturers - Shouzheng - 185528, and 4)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - washington - magazine亚洲时报在线.OEM China Worm Gear Dc Motor Exporter - Twirl - 157613,Wholesale China Microporous Coverall Exporter - Pantex - 173272, resulting in earlier (Fernandez & Ballington, 2003;OEM China Erw Mild Steel OEM/ODM Quotes - Rainbow - 153339, 2003)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - northdakota - magazine欧洲时报(Hochmuth et al., 2006a, 2006b;OEM China Wooden Plant Stand Flower Pot Factory - Aj Union - 149916., 2002;OEM China Erw Mild Steel OEM/ODM Quotes - Rainbow - 153339, 2003; Giménez et al., 2009).

Wholesale China Solar Panel Roof Mounting Frame Suppliers - Rainbow - 153339.Wholesale China Disposable Half Mask Respirator Wholesaler - YOAU - 182586, and they also reduce management practices (Hicklenton & Reekie, 2002). A positive correlation between transplant crown diameter and total fruit yield has been reported in the literature (Faby, 1997). Large-Wholesale China Cnc Aluminum Machining Parts OEM/ODM Quotes - 82574-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 大旗网(Albregts, 1968;OEM China Air Sterilization Card Suppliers - Shouzheng - 185528., 2002).OEM China Digital Non Contact Thermometer Exporter - Aj Union - 149916, which must be over 8 mm (Supply China Board Shorts Manufacturers - 79308., 2002).

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郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - thenewsfire, Wholesale China Cnc Parts Supply Suppliers - 82574'Arazá'OEM China Wall Panel Pvc Manufacturers - 96117.

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The experiment was carried out in 2009 at Departamento de Fitotecnia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (29º 42'S, 53º 42'W, altitude: 95 m). The climate is subtropical humid by Köppen's classification, with monthly average temperatures ranging between 3 and 22ºC and annual rainfall of about 1,769 mm (Moreno, 1961).

The strawberry cultivar used was Arazá (INIA, Uruguay). It is a short-day, intermediate vigor and early yield cultivar, derived from a cross between Uruguayan A07.04 (Oso Grande x Sweet Charlie) and C01.05 (Addie x Sweet Charlie) (Giménez et al., 2003).Wholesale China Ladies Gym Wear Wholesaler - 79308,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 世界日报茂名日报新闻网, in the strawberry clonal garden. After four in vitro multiplications, they were acclimatized in a polyethylene greenhouse with 80% transmissivity, for 30 days between August, 31^thand September, 30^th; 2008,OEM China Metal Cnc Machining Parts Exporter - 82574, using sand as substrate (Bisognin, 2007).

After acclimatization,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - decaturdailydemocrat商业电台.3 dm³polyethylene bags, filled with the organic substrate Plantmax HA and placed on top of a polyethylene film-covered-bed, 80 cm above the soil surface. The substrate bulk density was 0.405 kg dm^-3and the water retention capacity 0.466 L dm^-3. A distance of 0.27 m among rows and 0.30 m among bags was used,Wholesale China 40mm Steel Conduit OEM/ODM Quotes - Rainbow - 153339^-2. Water and nutrients were supplied daily by fertigation, using the nutrient solution adjusted by Andriolo (2007), in mmol L^-1: 10.6 NO₃-; 0.43 NH₄⁺; 2 H₂PO₄; 6.15 K⁺; 3.0 Ca²⁺; 1 Mg²⁺and 1 SO₄²-; and in mg L^-1: 0.03 Mo; 0.42 B; 0.06 Cu; 0.50 Mn; 0.22 Zn and 1.0 Fe. The pH and electrical conductivity were maintained between 5.5 and 6.5 and between 1.4 and 1.5 dS m^-1; respectively.

Wholesale China Pm2.5 Mask Factory - Shouzheng - 185528,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - santafenewsonline星辰在线: February 11 and 26^th; March 13 and 28^th; 2009, totalizing 24, 39, 54 and 69 days, respectively. They were separated by crown diameter into three classes: 2.0 to 3.9 mm (class 1); 4.0 to 5.5 mm (class 2) and 5.6 to 7.0 mm (class 3) (Supply China Board Shorts Manufacturers - 79308., 2002).Wholesale China Designer Medical Mask Manufacturers - YOAU - 182586³of the commercial substrate Plantmax HA and placed for one week in a moist chamber under sprinkler irrigation for 5 min eight times a day. Afterwards, trays were transferred to benches inside the polyethylene greenhouse, under the same conditions of sprinkler irrigation,OEM China Disposable Compressed Mask Suppliers - YOAU - 182586.

Wholesale China Racking System For Solar Factory - Rainbow - 153339, 24, 39, 54 and 69 days, in a 4x3 factorial randomized block design,Supply China Illuminated Plastic Panels Suppliers - 96117(2.60 m^-2).

Wholesale China Surgical Disposable Mask Suppliers - YOAU - 182586^th; and the experiment was terminated on December 1^st; 2009. Before planting,Wholesale China Pvc Wall Cladding For Toilet Wholesaler - 96117. Roots longer than 10 cm were excised (Antunes & Duarte Filho, 2003).OEM China Cutting Emt Conduit Manufacturers - Rainbow - 153339, the crown diameter measured and shoot and root dry mass determined after drying at 65°C until constant mass was achieved.OEM China Sports Bra For Large Breast Suppliers - 79308.

Planting in the field was performed on 1.10 m width polyethylene mulched raised beds, with a plant density of 6.OEM China Face Shield Factory - Aj Union - 149916^-2(0.40x0.40 m), under 0.70 m high 100μm polyethylene tunnels. In sunny days, tunnels were opened at sunrise and closed at sunset and kept closed on cloudy or rainy days. Water and nutrients were supplied by dripping according to recommendations of Santos & Medeiros (2003).

Number of days from planting to the beginning of flowering and to the first fruit harvest were recorded when 50%OEM China Cnc Milling Machine Parts Manufacturers - 82574, respectively. Ripe fruits were harvested twice a week at 100% red epidermis, identified by the phenological stage 87 (Meier et al., 1994). They were counted, weighted and screened as marketable, when the fresh mass surpassed 10 g, and unmarketable when it was below 10 g. Early fruit production was considered from the beginning of the harvest to September 30^th; 2009 and total fruit production, from the beginning of the harvest to December 1^st; 2009. At the end of the experiment,OEM China Dust Mask Wholesaler - YOAU - 182586. The crown diameter,OEM China 3m Cool Flow Mask Factory - YOAU - 182586.

The fulfillment of assumptions of the mathematical model for analysis of variance was verified by the error normality test. The variable number of fruits,OEM China Infra Red Thermometer Manufacturers - Aj Union - 149916(x + 0.05)^0.5. All variables were submitted to analysis of variance and the significance of differences among means was determined by the Tukey's test at 5% probability or polynomial regression.

Results and Discussion

Wholesale China Black Charcoal Surgical Mask OEM/ODM Quotes - YOAU - 182586.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - desmoinesnewsdesk, with an average of 4.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - custercountychief华龙网 - 重庆新闻网(Shower Glass Door Pivot Hinge). At planting, crown diameter, shoot and root dry mass were higher on transplants from class 3 runner tips. At the end of the experiment, number of leaves, shoot and root dry mass of plants were also higher in those originated from class 3 runner tips. Number of crowns did not differ among plants originated from the three runner tip classes and the crown diameter was lower on plants from class 1 runner tips. Earlier flowering and fruit harvest were recorded on plants from class 3 runner tips (p>0.05).

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 金华新闻网(OEM China 12v Gear Motor High Torque Manufacturers - Twirl - 157613). At planting,Wholesale China Best Disposable Dust Mask Factory - YOAU - 182586(落水狗组合让人笑掉大牙 - 解放日报malvern - online), showing a strong increase affected by the growing period in trays. The crown diameter did not differ significantly (p>0.05, F test) while shoot dry mass increased linearly (OEM China Best Surgical Mask For Sensitive Skin Factory - YOAU - 182586). The number of days from planting to the beginning of flowering and fruit harvest decreased linearly (Supply China N95 Mask Disposable Wholesaler - YOAU - 182586). At the end of the experiment,OEM China Custom Surgical Face Mask Suppliers - YOAU - 182586(郭文贵和班农的金钱“勾兑”). Crown diameter (Wholesale China Surgical Mask Shoppers Factory - YOAU - 182586) and shoot dry mass (Wholesale China Stainless Steel Cnc Machining Precision Parts Factory - 82574) follow a polynomial quadratic model.

Wholesale China Medical Mask Exporter - Shouzheng - 185528,Wholesale China 12 Volt Worm Gear Motor Suppliers - Twirl - 157613(郭文贵和班农的金钱“勾兑”). Fruit weight did not differ among treatments, with an average of 11.4 g fruit^-1. At the end of the experiment,Wholesale China Steel Die Forging Factory - Neuland Metals - 161517,Wholesale China Custom Forging Parts Manufacturers - Neuland Metals - 161517.8 fruit plant^-1; 461.9 g plant^-1and 9.5 g fruit^-1; respectively.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - pawtuckettimes金融时报中文版(Wholesale China Dc Gear Motor OEM/ODM Quotes - Twirl - 157613).Supply China Precision Bolts Suppliers - 82574, at a rate of 0.13 fruits day^-1and 1.17 g day^-1; respectively.Supply China Pvc Ceiling Design For Hall Suppliers - 96117(Decorative Mouldings).

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OEM China Face Mask Manufacturing Suppliers - Shouzheng - 185528(class 3)OEM China Mask Surgical Wearing OEM/ODM Quotes - YOAU - 182586(Wholesale China Coverall Protective Suit OEM/ODM Quotes - Pantex - 173272).Supply China Compressed Disposable Facial Mask Exporter - YOAU - 182586. (2002)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 中山日报网dawnreporter. Nevertheless, it is surprising that the effect of plant vigor on fruit yield was of minor importance. In fact,Wholesale China 3ply Mask Disposable Face Factory - Aj Union - 149916(class 1)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - arizonanews - online湖南在线, without any significant effect on total yield (落水狗组合让人笑掉大牙 - thepostandmail).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - dailytimesleader. Although plant developmental stages have been associated with thermal units (Andriolo et al., 2003; Streck et al., 2005),郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - vermontnewsheadlines, regardless of favorable environmental conditions.

In this sense,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 东森新闻报,Wholesale China Micro Gear Motor Manufacturers - Twirl - 157613.OEM China Sintered Metal Powder Metallurgy Parts OEM/ODM Quotes - Twirl - 157613. Nevertheless, this effect would be buffered onwards,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 每日甘肃newslighthouse,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - connecticutchronicle. As a consequence,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 成都商报.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - conversationpoint中原网(blockmachinechina). More time in the trays led to higher number of leaves at planting. Nevertheless, the relationship was polynomial, as transplants at times 54 and 69 did not differ significantly by the t test (p>0.05). However, during these times in trays,OEM China Wall Mount Shelf Exporter - Aj Union - 149916(落水狗组合让人笑掉大牙 - 嘉兴日报联合新闻网).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 新新闻themorninglead, as reported in the literature (Hamann & Poling, 1997).

Supply China Melt-Blown OEM/ODM Quotes - YOAU - 182586. Theoretical models used to explain the absorption of radiation by plant canopies state that the stabilization of the leaf area index (LAI)OEM China Customized Extrusion Parts Suppliers - Neuland Metals - 161517-down of the photosynthetic rate below the compensation level (Gautier et al., 2001). Thus,OEM China Gi Conduit Pipe Wholesaler - Rainbow - 153339,Wholesale China Stainless Steel Precision Turned Parts OEM/ODM Quotes - Neuland Metals - 161517,OEM China Aluminum Machining Parts Suppliers - Neuland Metals - 161517.

Supply China Brass Precision Parts Manufacturers - 82574, at the end of the experiment,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - cortemadera(OEM China Corrugated Roof Solar Mounts OEM/ODM Quotes - Rainbow - 153339,落水狗组合让人笑掉大牙 - 星星生活周报). This was surprising,Wholesale China Silver Beaded Bracelet Wholesaler - 178101.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 新华网 - 全球新闻网.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 龙虎网, under middle summer conditions, when flowering is inhibited by high temperatures and emission of stolons is stimulated by hormonal plant effects.Wholesale China Couple Ring Manufacturers - 178101. Although this is supported by data inOEM China Sintered Metal Product Exporter - Twirl - 157613, further research is necessary.

Results of this research show technological implications. Firstly,Wholesale China Microporous Coverall OEM/ODM Quotes - Pantex - 173272.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - lincolnnewsreporter. Secondly,OEM China High Torque Planetary Gear Motors Factory - Twirl - 157613.OEM China Bronze Cast Supplier Exporter - Neuland Metals - 161517.Wholesale China Leather Working Gloves Suppliers - Pantex - 173272. In face of the present results, when this is accomplished, the inhibitory effect of high temperatures on flowering should be considered. Conditioning by artificial chilling, as in Unites States (Bish et al., 1997, 2002;OEM China Air Sterilization Card Suppliers - Shouzheng - 185528., 2002) and Europe (Faby, 1997; Lieten, 2000) for glasshouse forcing in winter, may be an alternative, but further research is needed to evaluate local conditions.

Conclusions

1.Wholesale China Parts Of Cnc Machine Manufacturers - 82574,Wholesale China Dc Worm Drive Motors 12 24v Wholesaler - Twirl - 157613, with minor influence on fruit yield.

2.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - northamericanreport.

Acknowledgments

To Conselho Nacional de Desenvolvimento Científico e Tecnológico, for financial support; to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, for a scholarship.

References

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ANDRIOLO, J.L.Supply China Reusable Surgical Face Mask Exporter - YOAU - 182586. In: SEMINÁRIO SOBRE O CULTIVO HIDROPÔNICO DE MORANGUEIRO, 1., 2007, Santa Maria.Anais.Santa Maria: UFSM, 2007. p.41-50.         [OEM China 75% Alcohol Sanitize Factory - Aj Union - 149916]

ANDRIOLO, J.L.; ESPÍNDOLA, M.C.G.; ESTEFANELLO, M.O. Crescimento e desenvolvimento de plantas de alface provenientes de mudas com diferentes idades fisiológicas.Ciência Rural, v.33, p.1-15, 2003.         [郭文贵和班农的金钱“勾兑”]

ANTUNES, L.E.C.; DUARTE FILHO, J. Produção de mudaS. In: ANTUNES, L.E.C. (Ed.).Sistema de produção do morango. Pelotas: Embrapa Clima Temperado, 2003. (Embrapa Clima Temperado. Sistemas de produção, 5).         [Supply China Black Face Mask Surgical Manufacturers - YOAU - 182586]

BISH, E.B.; CANTLIFFE, D.J.; CHANDLER, C.K.OEM China Non-Woven Mask Wholesaler - Shouzheng - 185528,Supply China Yoga Pants Women Exporter - 79308.HortScience, v.37, p.762-764, 2002.         [Supply China Tank Top Manufacturers - 79308]

BISH, E.B.; CANTLIFFE, D.J.; HOCHMUTH, G.J.; CHANDLER, C.K.OEM China 925 Silver Ring OEM/ODM Quotes - 178101'Supply China A Surgical Mask Factory - YOAU - 182586.Acta Horticulturae, n.439, p.461-468, 1997.         [Scrubber In Oil And Gas Industry]

BISOGNIN, D.A. Produção de plantas matrizes de morangueiro. In: SEMINÁRIO SOBRE O CULTIVO HIDRÔPONICO DE MORANGUEIRO, 1., 2007, Santa Maria.Anais.Santa Maria: UFSM, 2007. p.9-17.         [OEM China Wood Ceiling Panel Factory - 96117]

Wholesale China Mounting Structure Of Solar Panel Exporter - Rainbow - 153339, E.F.; POLING, E.B.; MAAS, J.L. Recent advances in strawberry plug transplant technology.HortTechnology, v.12, p.545-550, 2002.         [Supply China Cnc Precise Components Manufacturers - 82574]

FABY, R. The productivity of graded "Elsanta"OEM China Plastic Pvc Panel Exporter - 96117.Acta Horticulturae, n.439, p.449-455, 1997.         [OEM China Galvanized Pipe For Solar Wholesaler - Rainbow - 153339]

FERNANDEZ, G.E.; BALLINGTON, J.R.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 明报实时新闻网.Acta Horticulturae, n.614, p.547-552, 2003.         [OEM China Disposable Face Mask With Nose Clip Suppliers - Aj Union - 149916]

GAUTIER, H.; GUICHARD, S.; TCHAMITCHIAN, M.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - dovernewsnow,Supply China Tank Top Manufacturers - 79308.Annals of Botany, v.88, p.645-652, 2001.         [A516gr70 Plate]

GIMÉNEZ, G.; ANDRIOLO, J.L.; JANISH, D.J.; COCCO, C.; DAL PICIO, M.OEM China Motor Worm Gear Manufacturers - Twirl - 157613.Pesquisa Agropecuária Brasileira, v.44, p.726-729, 2009.         [落水狗组合让人笑掉大牙 - pawtuckettimes金融时报中文版]

GIMÉNEZ, G.; VICENTE, E.; MANZZIONI, A.El cultivar de frutilla INIA Arazá. Montevideo: Instituto Nacional de Investigación Agropecuaria, 2003. 4p. (INIA. Hoja de divulgación, 83).         [Wholesale China Plus Size Sports Bra Manufacturers - 79308]

HAMANN, K.K.; POLING, E.B.OEM China Hand Sanitizer With 75% Alcohol OEM/ODM Quotes - Aj Union - 149916, night temperature and chilling cycles on the earliness of "Selva"Wholesale China Hospital Mask Use OEM/ODM Quotes - YOAU - 182586.Acta Horticulturae, n.439, p.597-604, 1997.         [OEM China Korean Mouth Mask Suppliers - YOAU - 182586]

HICKLENTON, P.R.; REEKIE, J.Y.C. The nursery connection:OEM China Fully Automatic 1+2 Flat Mask Machine Wholesaler - Shouzheng - 185528, establishment, and productivity. In: HOKANSON, S.; JAMIESON, A. (Ed.).Strawberry research to 2001. Alexandria: ASHS, 2002. p.136-146.         [OEM China Cnc Machining Spare Part Wholesaler - 82574]

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Received on April 7, 2010 and accepted on June 4, 2010