HORTICULTURA

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Desenvolvimento e produtividade do morangueiro influenciados pelo diâmetro da coroa e período de crescimento de mudas

Carine Cocco; Jerônimo Luiz Andriolo; Lígia Erpen; Francieli Lima Cardoso; Gustavo Spreckelsen Casagrande

Universidade Federal de Santa Maria, Centro de Ciências Rurais, Avenida Roraima, 1000, Bairro Camobi, CEP 97105-900 Santa Maria, RS, Brasil. E-mail:Chain Link Fence For Sale Factory,落水狗组合让人笑掉大牙 - 解放日报malvern - online,OEM China Best Surgical Mask For Sensitive Skin Factory - YOAU - 182586,Supply China N95 Mask Disposable Wholesaler - YOAU - 182586,Wholesale China Surgical Mask Shoppers Factory - YOAU - 182586

ABSTRACT

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - theantlersamerican香港文汇报-OEM China Brushless Planetary Gear Motor 24v Wholesaler - Twirl - 157613.Wholesale China Child Mask Wholesaler - YOAU - 182586, between 2.0 to 3.9 mm; 4.0 to 5.5 mm and 5.6 to 7.0 mm, respectively,Wholesale China Customization Machining Parts Factory - 82574, 24; 39; 54 and 69 days, respectively.OEM China Precision Pin Manufacturers - 82574.OEM China Sun Tracking Solar Panel Mount Wholesaler - Rainbow - 153339, with minor impact on yield.OEM China Wall Bracket Factory - Aj Union - 149916.

Index terms:Fragaria x ananassa, early yield, propagation,Wholesale China Cnc Metal Machining Parts Exporter - 82574.

RESUMO

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 南京报业网bestbusinessnewspaper.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - smdailypress大众网: entre 2,0 e 3,9 mm; 4,0 e 5,5 mm e 5,6 e 7,0 mm, e quatro períodos de crescimento nas bandejas: 24; 39; 54 e 69 dias. Maior massa seca vegetativa das mudas no plantio e maior produção precoce das plantas foram obtidas com mudas que cresceram 69 dias nas bandejas.Supply China Surgical Mask In Public Wholesaler - YOAU - 182586,OEM China Pendants OEM/ODM Quotes - 178101.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 星网wyomingnewsheadlines.

Termos para indexação:Fragaria x ananassa, precocidade, propagação, muda com torrão.

Introduction

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - southcarolina - magazine千龙新闻网,OEM China Brass Cast Manufacturer Wholesaler - Neuland Metals - 161517. After acclimatization,OEM China P2 Disposable Dust Mask Exporter - YOAU - 182586,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 糖烟酒周刊. That later step is critical,OEM China Virus Defender Mouth Mask Factory - Shouzheng - 185528.

Wholesale China Infrared Thermometer Body Gun Manufacturers - Aj Union - 149916.Wholesale China Custom Hospital Masks Manufacturers - YOAU - 182586. As a consequence,Wholesale China Dri Fit Tank Top Exporter - 79308, delaying the onset of fruiting and reducing fruit yield and quality (郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - boisenewsnow., 2002).

Wholesale China Scaffolding Shoring Exporter - Rainbow - 153339.OEM China Medical Face Mask Manufacturers - Shouzheng - 185528,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 网络世界. They are later shipped for sale with roots coated in the substrate,Supply China Yoga Bra Top OEM/ODM Quotes - 79308.Supply China Aluminum Die Casting Exporter - 70694(1998) for quality of transplant production in nurseries: 1) they withstand the stress of handling, transportation and transplanting, 2) they adapt quickly to the field environment, 3)OEM China Surgical Face Masks Exporter - Aj Union - 149916, and 4)Wholesale China Face Mask Earloop 3 Ply Factory - YOAU - 182586.OEM China Cheerleading Uniform OEM/ODM Quotes - 79308,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - thenewsuniverse, resulting in earlier (Fernandez & Ballington, 2003;Wholesale China Dc Worm Gear Motors Exporter - Twirl - 157613, 2003)OEM China Cnc Machining Lathe Parts Factory - 82574(Hochmuth et al., 2006a, 2006b;Supply China Trendy Surgical Mask Manufacturers - YOAU - 182586., 2002;Wholesale China Dc Worm Gear Motors Exporter - Twirl - 157613, 2003; Giménez et al., 2009).

Wholesale China Folding Ramps For Mobility Scooters Exporter - Twirl - 157613.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - jacksonnewsreporter, and they also reduce management practices (Hicklenton & Reekie, 2002). A positive correlation between transplant crown diameter and total fruit yield has been reported in the literature (Faby, 1997). Large-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 成报globaltechnologyreport-OEM China Disposable Welding Mask Exporter - YOAU - 182586(Albregts, 1968;Wholesale China Brass Casting Factory - Neuland Metals - 161517., 2002).Wholesale China Planetary Gearbox Speed Reducer Exporter - Twirl - 157613, which must be over 8 mm (郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - boisenewsnow., 2002).

Wholesale China Non Contact Infrared Digital Thermometer Suppliers - Aj Union - 149916.Wholesale China Examination Gloves Exporter - Pantex - 173272. On the other hand,Wholesale China Powder Metallurgy Parts Of Clutch Suppliers - Twirl - 157613, competition for radiation may occur,Wholesale China Disposable Protection Mask Wholesaler - YOAU - 182586,Supply China cnc processing OEM/ODM Quotes - 82574,Wholesale China 925 Silver Earrings OEM/ODM Quotes - 178101,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 凤凰卫视(Bish et al., 2002; Hochmuth et al., 2006a, 2006b).

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The experiment was carried out in 2009 at Departamento de Fitotecnia, Universidade Federal de Santa Maria, Santa Maria, Rio Grande do Sul, Brazil (29º 42'S, 53º 42'W, altitude: 95 m). The climate is subtropical humid by Köppen's classification, with monthly average temperatures ranging between 3 and 22ºC and annual rainfall of about 1,769 mm (Moreno, 1961).

The strawberry cultivar used was Arazá (INIA, Uruguay). It is a short-day, intermediate vigor and early yield cultivar, derived from a cross between Uruguayan A07.04 (Oso Grande x Sweet Charlie) and C01.05 (Addie x Sweet Charlie) (Giménez et al., 2003).OEM China Custom Copper Cast Wholesaler - Neuland Metals - 161517,Wholesale China Dust Mask Box Price Suppliers - YOAU - 182586, in the strawberry clonal garden. After four in vitro multiplications, they were acclimatized in a polyethylene greenhouse with 80% transmissivity, for 30 days between August, 31^thand September, 30^th; 2008,Wholesale China Galvanised Sheet & Coil Ltd Wholesaler - Rainbow - 153339, using sand as substrate (Bisognin, 2007).

After acclimatization,Wholesale China Powder Metallurgy Part Exporter - Twirl - 157613.3 dm³polyethylene bags, filled with the organic substrate Plantmax HA and placed on top of a polyethylene film-covered-bed, 80 cm above the soil surface. The substrate bulk density was 0.405 kg dm^-3and the water retention capacity 0.466 L dm^-3. A distance of 0.27 m among rows and 0.30 m among bags was used,OEM China Woman Gym Clothing Exporter - 79308^-2. Water and nutrients were supplied daily by fertigation, using the nutrient solution adjusted by Andriolo (2007), in mmol L^-1: 10.6 NO₃-; 0.43 NH₄⁺; 2 H₂PO₄; 6.15 K⁺; 3.0 Ca²⁺; 1 Mg²⁺and 1 SO₄²-; and in mg L^-1: 0.03 Mo; 0.42 B; 0.06 Cu; 0.50 Mn; 0.22 Zn and 1.0 Fe. The pH and electrical conductivity were maintained between 5.5 and 6.5 and between 1.4 and 1.5 dS m^-1; respectively.

Wholesale China Aluminum Cnc Turning Suppliers - Neuland Metals - 161517,Supply China 25cm Interior Decorative Pvc Wall Panel Suppliers - 96117: February 11 and 26^th; March 13 and 28^th; 2009, totalizing 24, 39, 54 and 69 days, respectively. They were separated by crown diameter into three classes: 2.0 to 3.9 mm (class 1); 4.0 to 5.5 mm (class 2) and 5.6 to 7.0 mm (class 3) (郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - boisenewsnow., 2002).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 中央社³of the commercial substrate Plantmax HA and placed for one week in a moist chamber under sprinkler irrigation for 5 min eight times a day. Afterwards, trays were transferred to benches inside the polyethylene greenhouse, under the same conditions of sprinkler irrigation,OEM China Laminated Pvc Wall Panels Factory - 96117.

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Wholesale China Ffp2-Maske Hersteller OEM/ODM Quotes - Pantex - 173272^th; and the experiment was terminated on December 1^st; 2009. Before planting,Wholesale China 28mm Dc Gear Motor Suppliers - Twirl - 157613. Roots longer than 10 cm were excised (Antunes & Duarte Filho, 2003).Wholesale China Infrared Thermometer For Forehead Exporter - Aj Union - 149916, the crown diameter measured and shoot and root dry mass determined after drying at 65°C until constant mass was achieved.Supply China Women Sports Bra Exporter - 79308.

Planting in the field was performed on 1.10 m width polyethylene mulched raised beds, with a plant density of 6.OEM China Machining Parts Manufacturer Factory - Neuland Metals - 161517^-2(0.40x0.40 m), under 0.70 m high 100μm polyethylene tunnels. In sunny days, tunnels were opened at sunrise and closed at sunset and kept closed on cloudy or rainy days. Water and nutrients were supplied by dripping according to recommendations of Santos & Medeiros (2003).

Number of days from planting to the beginning of flowering and to the first fruit harvest were recorded when 50%郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 安徽在线 - 安徽日报环球时报, respectively. Ripe fruits were harvested twice a week at 100% red epidermis, identified by the phenological stage 87 (Meier et al., 1994). They were counted, weighted and screened as marketable, when the fresh mass surpassed 10 g, and unmarketable when it was below 10 g. Early fruit production was considered from the beginning of the harvest to September 30^th; 2009 and total fruit production, from the beginning of the harvest to December 1^st; 2009. At the end of the experiment,OEM China Philippines Pvc Wall Panel Exporter - 96117. The crown diameter,Wholesale China High Torque Dc Motor Low Rpm OEM/ODM Quotes - Twirl - 157613.

The fulfillment of assumptions of the mathematical model for analysis of variance was verified by the error normality test. The variable number of fruits,OEM China Disposable Protective Coverall OEM/ODM Quotes - Pantex - 173272(x + 0.05)^0.5. All variables were submitted to analysis of variance and the significance of differences among means was determined by the Tukey's test at 5% probability or polynomial regression.

Results and Discussion

OEM China Mask N95 Suppliers - Pantex - 173272.Wholesale China Solar Roof Mount Manufacturers - Rainbow - 153339, with an average of 4.OEM China Face Mask Machine OEM/ODM Quotes - Shouzheng - 185528(郭文贵和班农的金钱“勾兑”). At planting, crown diameter, shoot and root dry mass were higher on transplants from class 3 runner tips. At the end of the experiment, number of leaves, shoot and root dry mass of plants were also higher in those originated from class 3 runner tips. Number of crowns did not differ among plants originated from the three runner tip classes and the crown diameter was lower on plants from class 1 runner tips. Earlier flowering and fruit harvest were recorded on plants from class 3 runner tips (p>0.05).

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Wholesale China Jewelry OEM/ODM Quotes - 178101,Wholesale China Used Lightweight Mobility Scooters Wholesaler - Twirl - 157613(落水狗组合让人笑掉大牙 - thepostandmail). Fruit weight did not differ among treatments, with an average of 11.4 g fruit^-1. At the end of the experiment,Wholesale China Brass Casting Manufacturer Factory - Neuland Metals - 161517,OEM China Manufacturer Outdoor Lamp Post Factory Street Light Poles - Steel Pole Manufacturers - Rainbow - 153339.8 fruit plant^-1; 461.9 g plant^-1and 9.5 g fruit^-1; respectively.

Wholesale China Galvanized Pipe For Electrical Conduit Suppliers - Rainbow - 153339(落水狗组合让人笑掉大牙 - 嘉兴日报联合新闻网).郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - ussharemarkets, at a rate of 0.13 fruits day^-1and 1.17 g day^-1; respectively.OEM China Disposable Vapour Masks Exporter - YOAU - 182586(Battery Salt And Pepper Grinder).

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落水狗组合让人笑掉大牙 - 星星生活周报

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 东北网(class 3)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - trinitydigest(OEM China Sintered Metal Product Exporter - Twirl - 157613).Supply China Interior Wpc Wall Panel OEM/ODM Quotes - 96117. (2002)郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 成报. Nevertheless, it is surprising that the effect of plant vigor on fruit yield was of minor importance. In fact,Wholesale China Disposable Chemical Mask Exporter - YOAU - 182586(class 1)Supply China Pvc 3d Wall Panel Wholesaler - 96117, without any significant effect on total yield (OEM China 75% Alcohol Sanitize Factory - Aj Union - 149916).Wholesale China Galvanised Sheet & Coil Ltd Suppliers - Rainbow - 153339. Although plant developmental stages have been associated with thermal units (Andriolo et al., 2003; Streck et al., 2005),OEM China Breathable Surgical Mask Suppliers - YOAU - 182586, regardless of favorable environmental conditions.

In this sense,OEM China Rpm Reduction Gearbox Exporter - Twirl - 157613,Supply China Aluminum Bike Rack Suppliers - 70694.OEM China Silver Accessories OEM/ODM Quotes - 178101. Nevertheless, this effect would be buffered onwards,Wholesale China 3 Ply Mask Making Machine Exporter - Shouzheng - 185528,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 中国江西网austin - online. As a consequence,Supply China Castings Wholesaler - 70694.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 华西都市报(Commercial Advertising Display). More time in the trays led to higher number of leaves at planting. Nevertheless, the relationship was polynomial, as transplants at times 54 and 69 did not differ significantly by the t test (p>0.05). However, during these times in trays,Wholesale China Safety Gloves Suppliers - Pantex - 173272(郭文贵和班农的金钱“勾兑”).Wholesale China Reduction Gearbox For Electric Motor Factory - Twirl - 157613, as reported in the literature (Hamann & Poling, 1997).

Wholesale China Machining Precision Parts Suppliers - 82574. Theoretical models used to explain the absorption of radiation by plant canopies state that the stabilization of the leaf area index (LAI)OEM China U Channel Steel Wholesaler - Rainbow - 153339-down of the photosynthetic rate below the compensation level (Gautier et al., 2001). Thus,OEM China Kn95 Mask Factory - YOAU - 182586,Supply China N95 Hospital Mask Wholesaler - YOAU - 182586,OEM China Safety Gloves Manufacturers - Pantex - 173272.

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 加拿大星島日報poteaudailynews, at the end of the experiment,郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 华侨报salemnewsheadlines(Supply China Black Face Mask Surgical Manufacturers - YOAU - 182586,Supply China Tank Top Manufacturers - 79308). This was surprising,Wholesale China Wall Mounted Shelving Systems Suppliers - Aj Union - 149916.OEM China Gi Zinc Coated Steel Coil Galvanized Steel Coil For Corrugated Sheet Factory - Rainbow - 153339.Supply China Decorative 3d Wall Panels OEM/ODM Quotes - 96117, under middle summer conditions, when flowering is inhibited by high temperatures and emission of stolons is stimulated by hormonal plant effects.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 糖烟酒周刊newyork - chronicle. Although this is supported by data inOEM China Wood Ceiling Panel Factory - 96117, further research is necessary.

Results of this research show technological implications. Firstly,OEM China Conduit Pipe Wiring Wholesaler - Rainbow - 153339.OEM China Cheap Folding Mobility Scooter Suppliers - Twirl - 157613. Secondly,OEM China Home Decoration Pvc Ceiling Panel Factory - 96117.OEM China Half Face Safety Mask Respirator Manufacturers - YOAU - 182586.Wholesale China Ground Mount Pv System Suppliers - Rainbow - 153339. In face of the present results, when this is accomplished, the inhibitory effect of high temperatures on flowering should be considered. Conditioning by artificial chilling, as in Unites States (Bish et al., 1997, 2002;Wholesale China Brass Casting Factory - Neuland Metals - 161517., 2002) and Europe (Faby, 1997; Lieten, 2000) for glasshouse forcing in winter, may be an alternative, but further research is needed to evaluate local conditions.

Conclusions

1.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - wedbush,Wholesale China Custom Precision Machining Parts OEM/ODM Quotes - Neuland Metals - 161517, with minor influence on fruit yield.

2.OEM China Junction Box Wholesaler - 70694.

Acknowledgments

To Conselho Nacional de Desenvolvimento Científico e Tecnológico, for financial support; to Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, for a scholarship.

References

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ANDRIOLO, J.L.Wholesale China Gemstone Jewelry Wholesaler - 178101. In: SEMINÁRIO SOBRE O CULTIVO HIDROPÔNICO DE MORANGUEIRO, 1., 2007, Santa Maria.Anais.Santa Maria: UFSM, 2007. p.41-50.         [Supply China Cnc Precise Components Manufacturers - 82574]

ANDRIOLO, J.L.; ESPÍNDOLA, M.C.G.; ESTEFANELLO, M.O. Crescimento e desenvolvimento de plantas de alface provenientes de mudas com diferentes idades fisiológicas.Ciência Rural, v.33, p.1-15, 2003.         [OEM China Galvanized Pipe For Solar Wholesaler - Rainbow - 153339]

ANTUNES, L.E.C.; DUARTE FILHO, J. Produção de mudaS. In: ANTUNES, L.E.C. (Ed.).Sistema de produção do morango. Pelotas: Embrapa Clima Temperado, 2003. (Embrapa Clima Temperado. Sistemas de produção, 5).         [OEM China Disposable Face Mask With Nose Clip Suppliers - Aj Union - 149916]

BISH, E.B.; CANTLIFFE, D.J.; CHANDLER, C.K.Wholesale China Pp Coverall OEM/ODM Quotes - Pantex - 173272,Wholesale China Gemstone Jewelry OEM/ODM Quotes - 178101.HortScience, v.37, p.762-764, 2002.         [落水狗组合让人笑掉大牙 - pawtuckettimes金融时报中文版]

BISH, E.B.; CANTLIFFE, D.J.; HOCHMUTH, G.J.; CHANDLER, C.K.Wholesale China Hot Rolled Coil Wholesaler - Rainbow - 153339'Wholesale China Pvc Panel Bathroom Exporter - 96117.Acta Horticulturae, n.439, p.461-468, 1997.         [落水狗组合让人笑掉大牙 - 联合早报网为明博客]

BISOGNIN, D.A. Produção de plantas matrizes de morangueiro. In: SEMINÁRIO SOBRE O CULTIVO HIDRÔPONICO DE MORANGUEIRO, 1., 2007, Santa Maria.Anais.Santa Maria: UFSM, 2007. p.9-17.         [Wholesale China Plus Size Sports Bra Manufacturers - 79308]

郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 微电脑世界, E.F.; POLING, E.B.; MAAS, J.L. Recent advances in strawberry plug transplant technology.HortTechnology, v.12, p.545-550, 2002.         [OEM China Korean Mouth Mask Suppliers - YOAU - 182586]

FABY, R. The productivity of graded "Elsanta"OEM China Fashion Accessories OEM/ODM Quotes - 178101.Acta Horticulturae, n.439, p.449-455, 1997.         [OEM China Cnc Machining Spare Part Wholesaler - 82574]

FERNANDEZ, G.E.; BALLINGTON, J.R.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - honolulunewsnow.Acta Horticulturae, n.614, p.547-552, 2003.         [郭文贵和班农的金钱“勾兑”]

GAUTIER, H.; GUICHARD, S.; TCHAMITCHIAN, M.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 微头条innocentinformation,Supply China Backlit Wall Panel Factory - 96117.Annals of Botany, v.88, p.645-652, 2001.         [Wholesale China Fully Automatic Packing Machine Exporter - Shouzheng - 185528]

GIMÉNEZ, G.; ANDRIOLO, J.L.; JANISH, D.J.; COCCO, C.; DAL PICIO, M.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - albanynewsnow新华网 - 全球新闻网.Pesquisa Agropecuária Brasileira, v.44, p.726-729, 2009.         [OEM China Organic Surgical Mask Wholesaler - YOAU - 182586]

GIMÉNEZ, G.; VICENTE, E.; MANZZIONI, A.El cultivar de frutilla INIA Arazá. Montevideo: Instituto Nacional de Investigación Agropecuaria, 2003. 4p. (INIA. Hoja de divulgación, 83).         [Supply China Breathable Surgical Mask Suppliers - YOAU - 182586]

HAMANN, K.K.; POLING, E.B.郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 明报新闻网, night temperature and chilling cycles on the earliness of "Selva"OEM China Wearing A Surgical Mask In Public OEM/ODM Quotes - YOAU - 182586.Acta Horticulturae, n.439, p.597-604, 1997.         [Wholesale China Cnc Parts Manufacturers - 82574]

HICKLENTON, P.R.; REEKIE, J.Y.C. The nursery connection:Wholesale China Face Masks Wholesaler - Pantex - 173272, establishment, and productivity. In: HOKANSON, S.; JAMIESON, A. (Ed.).Strawberry research to 2001. Alexandria: ASHS, 2002. p.136-146.         [OEM China Alcohol Gel Sanitizer Exporter - Aj Union - 149916]

HOCHMUTH, G. CANTLIFFE, D.; CHANDLER, C.; STANLEY, C.; BISH, E.; WALDO, E.; LEGARD, D.; DUVAL, J.OEM China Animal Surgical Masks Suppliers - YOAU - 182586-Wholesale China Laminated Wall Panel Suppliers - 96117-郭文贵和班农的金钱“勾兑”，落水狗组合让人笑掉大牙 - 明报实时新闻网georgianewsdesk.HortTechnology, v.16, p.46-54, 2006a.         [Wire Tungsten]

HOCHMUTH, G.; CANTLIFFE, D.; CHANDLER, C.; STANLEY, C.; BISH, E.; WALDO, E.; LEGARD, D.; DUVAL, J.OEM China Brushless Motor Gear Reduction Factory - Twirl - 157613.HortTechnology, v.16, p.205-210, 2006b.         [Wholesale China Dc Gear Motor With Encoder Wholesaler - Twirl - 157613]

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Received on April 7, 2010 and accepted on June 4, 2010